You are investigating how the transcription factor protein BLUE enters into the nucleus to turn on gene transcription and expression. You introduce a green fluorescent protein (GFP) tagged version of BLUE1 (called BLUE1-GFP) into wildtype cells (wt) You also introduce BLUE1-GFP into two different mutant strains that lack proteins hypothesized to be involved in nuclear transport (AWELLa and AWELLß) You know from previous experiments that the addition of a GFP-tag to BLUE1 does not affect its structure or function. In each mutant strain, you examine localization of the BLUE1-GFP tagged protein and compare it to the localization of a fluorescent molecule (DAPI) that binds to DNA. you are able to generate a mutant cell line replacing the normal (wildtype) gene encoding Ran-GEF with a Ran-GEF lacking its NLS (Nuclear Localization Sequence) What effect would expression of this mutant Ran-GEF have on the subcellular localization of BLUE1-GFP?