If a DNA genome contains 22% T, what percent of C is present? You want to have E. coli take up your recombinant DNA vector you designed. What is the most likely negative consequence of forgetting to add an antibiotic to the nutrient-containing plate that you plan to grow E. coli on?
a. The restriction endonuclease will not be able to cut the vector or gene insert.
b. The gene of interest will not be properly ligated into the vector.
c. E. coli that did not take up your vector will grow on the plate.
e. No E. coli will grow on the plate.
f. Blunt ends, instead of sticky ends, will be formed in the vector and gene insert.
