You want to have E . coli take up your recombinant DNA vector you designed. What is the most likely negative consequence of forgetting to add an antibiotic to the nutrientcontaining plate that you plan to grow E . coli on ?
A. Blunt ends, instead of sticky ends, will be formed in the vector and gene insert.
B. The gene of interest will not be properiy ligated into the vector.
C. The restriction endonuclease will not be able to cut the vector or gene insert.
D. E . coli that did not take up your vector will grow on the plate.
E. No E . coli will grow on the plate.
