Determine the concentration of Amylase in your Saliva Samples
The standard curve can be used to determine the amylase concentration of your unknown samples since you measured the absorbance. The equation of the line in the format y = mx + b, can be used to calculate the unknown amylase concentration in your saliva. This can be accomplished by inputting the measured absorbance value of each unknown sample into the equation of the line and solving the equation to determine the amylase concentration in each of these tubes. Please remember to factor in the dilution of your saliva sample when calculating your Amylase conc (mg/ml) and if done correctly, your calculated concentration from the standard curve will be the same value for 10x, 100x and 1000x saliva samples. Please use your completed curve to determine the concentration of amylase in your unknown saliva samples. Please show your calculations for each dilution and your final calculated concentration to your TA.