A lab is interested in studying the human stem cell associated transcription factor, ZNF521. They want to study ZNF521 by creating a fusion protein where ZNF521 is tagged with the Flag protein (which is used as an epitope, similar to GST or other tags).
After constructing their ZNF521-Flag plasmid and transforming it into human cells, they want to verify that the fusion protein is expressed using a northern blot. The DNA sequence of the ZNF521 gene is below (assume for the sake of this question that the ZNF521 gene is only 10 nucleotides long):
The research team speculates that ZNF521 regulates expression of Oct4 and plans to examine this first by seeing whether ZNF521 protein binds to Oct4 promoter DNA in vitro. Which of the following techniques will enable them to examine this?
a. Q-RT PCR
b. EMSA
c. Immunoprecipitation
d. Microarray
