2. Consider this follow-up experiment: You take the bacteria which are living in the center of the gel (at the 1000x concentration), and kill them. You next take those killed bacteria and mix them in a test tube with a pool of sensitive bacteria taken taken from the "0" zone. After about an hour, you pour the mixture into a new resistance gel (set up the same way as the one in the video) and watch for growth. IF horizontal gene transfer (DNA transfer) has taken place, how would the growth on the gel appear? Would it be the same (or different) way as in the video? Would it take 11 days for the bacteria to reach the highest (1000x) concentration? Would there be pauses at each concentration interface? Explain your rationale. Lastly, if DNA transfer took place, which method is likely responsible (tranduction, conjugation, or transformation)? Discuss your answer by ruling out the methods not responsible.



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