Answer :
Final answer:
Biological monitoring, specifically using indicator spore tests like endospores of Geobacillus stearothermophilus, is the most reliable method to determine sterilization efficacy.
Explanation:
The best way to determine whether sterilization has occurred is by using biologic monitoring. Biological indicator spore tests, such as using endospores of Geobacillus stearothermophilus, are commonly employed to verify the effectiveness of sterilization processes. These tests involve incubating the indicators post-sterilization to ensure no viable endospores remain.
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